柱前衍生化HPLC法测定人纤维蛋白原中精氨酸的含量

Determination of arginine in human fibrinogen by HPLC with precolumn derivatization

目的：建立人纤维蛋白原中精氨酸的含量测定方法。方法：以丙氨酸为内标,2,4-二硝基氟苯(DN- FB)为柱前衍生剂,50%乙腈-0．05mol/L醋酸盐缓冲液(35∶65)为流动相,紫外362nm处检测。结果：精氨酸在0．16～0．80mg/mL浓度范围内线性关系良好(r=0．9998),平均空白加样回收率为102．4%,RSD为1．4%。结论：方法准确、简便,费用低,可用于人纤维蛋白原中精氨酸含量的控制。

Objective：To develop a method for determination of arginine in human fibrinogen. Methods：The sample was mixed with alanine as the internal standard, derived by 2,4-dinitrofluorobenzene. The mobil phase was composed of 50% acetonitrile- 0. 05 mol/L acetate buffer（ 35 ： 65 ）. The UV detection wavelength was 362nm. Results ：There was a good linear relationship with the range of 0. 16 - 0. 06mg/mL（r = 0. 999 8 ）, average recovery of arginine was 102.4% , RSD was 1.4%. Conclusion： This method is accurate, simple ,cheap, and can be used for determination of arginine in human fibrinogen.