毛细管电泳和高效液相作氨氯地平异构体分离分析研究

Separation of Enantiomer of Amlodipine by CE and HPLC

目的：毛细管电泳和高效液相作氨氯地平异构体分离分析研究。方法：毛细管电泳采用未涂渍熔融石英毛细管75μm×68cm(有效长度50cm)；优化选择浓度为100mmol/L的Tris缓冲液,含有1．0%羟丙基-β-CD(用磷酸调节pH至3．0)；分离电压18kV；温度15℃。高效液相色谱柱为ULTRON ES-OVM手性柱,流动相：乙腈-0．02mol/L磷酸二氢钠溶液(pH 7．0)(20∶80)；流速1．0ml/min。结果：氨氯地平对映异构体分离良好。结论：该方法简单可靠、专属性强。

Objective： Separation of enantiomer of amlodipine by CE and HPLC. Methods： Different separation conditions such as buffer pH, the concentration of the chiral selector, buffer concentrations, temperature and applied voltage, etc. were studied and optimized. The separation was finally conducted in 100mM orthophosphoric acid-Tris buffer （pH3.0） with 1.0% （w/w） HP-β-CD. The applied voltage was 16kV and the temperature 15℃. Results：Both CE and HPLC could give enantiomer of amlodipine resolution for adrenaline. Conclusion ：The method proved to be specific and accurate