梭曼对小鼠腹腔吞噬细胞的影响

Effect of Soman on Phagocytic Activity of peritoneal Cells in Mice

用化学发光法研究了Balb/c小鼠急性梭曼中毒后腹腔吞噬细胞吞噬活性的动态变化规律。154μg/kg梭曼皮下中毒后5～15min出现典型的胆碱能中毒症状,阵发性惊厥持续6～8h,24h死亡率为46％。中毒后第1天,腹腔吞噬细胞吞噬发光峰值由对照组的26.03±3.76(10~4/10~6个细胞)降至13.22±5.92;发光均值由对照组的0.10±0.02降至0.04±0.02,吞噬活性激发时间由3.8±1.1(s)延长至8.2±1.6,均与对照组有显著差异(P<0.05)。此后,三者与全血胆碱酯酶活力平行回升,发光均者与酶活力呈正相关(r=0.550,P<0.01)。毒扁豆碱合用阿托品预防,能有效缓解梭曼对吞噬细胞活性的抑制作用。

By using a chemiluminescence assay the effects of soman on phagocytic activities of mice peritoneal cells are investigated kinetically.Balb/c mice are used, soman (154 μg/kg sc) or physostigmine(100μg/kg sc) + atropine(17.4mg/kg sc) + soman (154μg/kg sc) are injected into the animals, 1 d after treatment with soman the peak value of chemiluminescence (count 104/106 cells) decreases from 26,03 ±3.76 to 13.22±5.92 and the mean value in every second is reduced from0.1± 0.02 to 0.04 ± 0.02 (P<0.05). Both the 2 parameters return slowly to the control levels on the 7th day. The phagocytic ability of peritoneal cells shows a positive relation to the blood cholinesterase activity.The prophylactic treatment with a combination of physostigmine and atropine can efficaciously diminish the toxic effect of soman.