大鼠心肌细胞葡萄糖-6-磷酸酶活性的超微结构定位

Ultrastructural Localization of Glucose-6-Phosphatasc Activity in Myocardial Cells of the Rats

本实验对大鼠心房及心室的心肌细胞葡萄糖-6-磷酸酶(G-6-Pase)活性进行了电镜观察,G-6-Pase反应产物见于肌浆网(SR)和核被膜内。我们发现心肌细胞内G-6-Pase活性呈现异质性分布,周围连接肌浆网(PJ-SR)和内部连接肌浆网(IJ-SR)内的G-6-pase反应产物较多,核被膜内含量较少,而网状肌浆网(N-SR)则含量稀少,或者缺乏。本文对心肌细胞内G-6-Pase的可能功能意义进行了讨论。

Glucose-6-phosphatase(G-6-Pase) activity in the atrial and ventricular muscle cells of the rats is examined by electron microscopy. The reaction product of G-6-Pase activity is present in the sarcoplasmic reticulum (SR) and the nuclear envelope of the myocardial cells. A heterogenous distribution of G-6-Pase activity in the cardial muscle cells is detected.In the myocardial cells the cytochemical reaction product for G-6-Pase activity is observed in a larger amount in the peripheral junctional sarcoplasmic reticulum(PJ-SR)and interior junctional SR(IJ-SR), and in smaller amounts in the nuclear envelope, is rarely present or absent in the network SR(N-SR).The possible functional significance of G-6-Pase in cardial muscle cells is discussed.