无细胞人胎肝制剂的成分及其对大鼠肝衰竭的疗效观察

Analysis of the Components of Cell-free Preparationsof Human Fetal Liver and Its Effect on Survivalof Experimental Hepatic Failure

本文观察了无细胞人胎肝制剂(胞浆液和透析液)及人胎朋细胞或成人肝细胞的胞浆液对实验性肝衰竭的治疗作用。结果发现:①胎肝胞浆液较其他3种制剂能显著提高肝衰竭大鼠存活率(P<0.01);②胎肝胞浆液治疗肝衰竭的关键性有效成分很可能是某些可溶性大分子活性物质,而非所含的激素、营养物质、微量元素、支链氨基酸等成分。

Effect of cell-free preparation of human fetal liver (cytosol and its dialysa-te) , human fetal muscle cytosol(FMC) and human adult liver cytosol (ALC) on survival rate of rats with acute hepatic failure induced by D-galactosamine was studied. The components of the 4 preparations were analysed preliminarily. Results: 1 ,fetal liver cytosol(FLC) could increase the survival rate(SR) of rats with hepatic failure(HF) comparing with normal saline(P<0.01), while the fetal liver dialysate(FLD), which contains no large molecule substances prepared from FLC, and FMC with almost the same protein contents as FLC hadn't the same effect(P>0.05) . The SR of rats with HF was reduced by ALC markedly) 2,IgG,IgA,IgM and C3 could not be detected in FLC, but a few kinds of hormones, trace elements, glucose and lipids, and biological large molecule substances (BLMS) such as nucleic acids and proteins were found. As to free amino acid,content of branch chain amino acids in FLC was significantly higher than that in ALC, but the content of aromatic amino acids and ammonia was markedly lower than that in ALC.The components in FLD were almost the same as in FLC except BLMS.These data suggest that the soluble BLMS in FLC may play a key role in treating HF. ALC could reduce SR and contained a large amount of poisonous substances such as aromatic amino acids and ammonia.