MMS对大鼠心肌毒性作用的研究

STUDY OF THE POISONOUS EFFECT OF MMS ON RAT'S MYOCARDIUM

用凝胶层析法分离出尿毒血症患者血浆中分子物质（ＭＭＳ），经尾静脉注入大鼠体内，观察ＭＭＳ对大鼠心肌收缩能力的影响。实验用左心室插管术，采集信号经换能器数字化后输入电子计算机，应用ＣＤＲＤＩＯ软件分析注射ＭＭＳ后心肌收缩力的改变。结果表明：ＭＭＳ可使心肌收缩能力减弱，心室内压下降（Ｐｅａｋ），室内压达峰值时间（ＰｅａｋＴｉｍｅ）推迟，室内压最大上升变化率减小ｄｐ／ｄｔｍａｘ，室内压下降最大变化率（－ｄｐ／ｄｔｍａｘ）变慢，室内压下降最大变化速率所在时间（－ｄｐ／ｄｔｍａｘＴｉｍｅ）延长，上述变化在注射ＭＭＳ后１ｍｉｎ即出现，持续１２ｍｉｎ左右恢复，说明ＭＭＳ对心肌有可逆性抑制作用。

Middle molecular substances(MMS) were gel-chromagraphically separated from the plasma of uraemia patients,and then injected into rats' tail veins to study the effect on myocardial contractility. Intubation was performed on the left ventricle of rats,the signal was sampled via a transducer, digitized, and then transferred into a microcomputer to be recorded and analyzed with CARDIO software. The results showed MMS could inhibit rats' myocardial contractility, lower the intraventricular pressure peak,delay the peak time,reduce both the maximum increasing rate(dp/dt max)and the decreasing rate(-dp/dt max) of intraventricular pressure and postpone the -dp/dt max time.These indexes began to alter I min after the injection of MMS,and lasted 12 min or so,then restored,which indicated the inhibition of myocardial contractility by MMS was reversible.