慢性乙肝肝纤维化不同分期TNF-α、NO和内毒素水平变化

Changes of TNF-α、NO and Endotoxin in Different Stages of Liver Fibrosis

探讨慢性乙肝肝纤维化不同分期TNF-α、NO和内毒素水平变化及其对肝纤维化的影响。经肝穿刺活检证实慢性乙肝肝纤维化患者100例,其中按S期又分为S120例、S225例、S330例、S425例;或按G期又分为G120例、G230例、G325例、G425例。对照组为20名健康人。所有患者肝穿刺的同时,取血清测NO、TNFα-和内毒素。结果表明:TNF-α随肝炎程度及肝纤维化程度加重而升高,相关r分别为0.623(P<0.05)、0.649(P<0.01),NO为0.328(P>0.05),内毒素在肝纤维化阶段尚无明显增高。结论:TNFα-在慢性乙肝发展至肝硬化过程中,始终起着重要作用;NO早期未参与肝硬化的形成与发展。

To discuss the alteration of cytokines in different stages of liver fibrosis and their influence on the formation and development of liver fibrosis. 100 patients with liver fibrosis had been demonstrated by liver biopsy and were classified under two types： as S1 20 cases ,S2 25 cases ,S3 30 cases and S4 25 cases;or G1 20 cases, G2 30 cases, G3 25 cases and G4 25 cases. 20 healthy subjects were in control group. At the same time of liver biopsy, serum was to be measured for NO, TNF-a, endotoxin. Results showed that TNF-a in hepatitis and liver fibrosis ,correlated with stages, r=0. 623（P〈0.05）, 0. 649 （P〈0.01） respectively, NO was not correlated r： 0. 328（P〉0.05）, endotoxin in liver fibrosis stage had not increased obviously. Endotoxin does not participate in chronic lesion of liver in early stage of the disease. In conclusion TNF-c~ plays an important into liver cirrhosis; NO has nothing to do with liver role all along in development of chronic hepatitis hardening in formation and development of liver cirrhosis in early stage.