微量量热法研究大肠杆菌对粉防己碱和粉防己总生物碱的耐药性

Drug Resistance of E.coli to Tetrandrine and Alkaloids of Stephenia tetrandra S.Moore by Microcalorimetry

从中药粉防己中提取出粉防己碱、粉防己总生物碱,应用微量量热法研究了它们对大肠杆菌的抑制作用以及两种药物作用下大肠杆菌的耐药性。结果表明,粉防己碱、粉防己总生物碱对大肠杆菌都有抑制作用,且粉防己碱比粉防己总生物碱的抑制效果好;大肠杆菌对粉防己碱、粉防己总生物碱都有耐药性,但大肠杆菌对粉防己总生物碱的耐药性产生慢。这可能是粉防己总生物碱中的复杂成分起了协同作用。

Tetrandrine（Tet） and alkaloids of Stephenia tetrandra S. Moore（AST） were isolated from Chinese natural medicine of Stephenia tetrandra S. Moore. The inhibitory action of Tet and AST and the drug resistance of E. coli to Tet and AST were studied by mieroealorimetric method. It was found that Tet and AST had inhibitory action on E. coli and the inhibitory action of Tet was better than that of AST. But the drug resistance of E. coli to AST was more slowly induced than that of Tet, which may be because the complicated components in AST could have a synergetic effect on inhibiting drug resistance.