摘要
目的观察依那普利和缬沙坦在降压的同时对自发性高血压大鼠(SHR)左室肥厚过程中心肌细胞凋亡及凋亡相关蛋白bc l-2、bax表达的影响。方法14周龄雄性SHR随机分为三组(n=6),依那普利组:依那普利30 mg.kg-1.d-1;缬沙坦组:缬沙坦30 mg.kg-1.d-1;对照组:等量饮用水灌胃,干预8周。分别采用流式细胞术Annexin V/PI法和免疫组化SABC法检测心肌细胞凋亡指数及凋亡相关蛋白bc l-2、bax的表达。结果缬沙坦及依那普利治疗组大鼠血压及左室重量/体重明显降低,心肌细胞凋亡指数明显降低(P<0.01);依那普利组明显降低bax蛋白表达,增加bc l-2蛋白表达(P<0.01)。缬沙坦组明显降低bax蛋白表达(P<0.01)。结论依那普利和缬沙坦对SHR左室肥厚过程中心肌细胞凋亡均有抑制作用。两者均通过增加bc l-2/bax比率而抑制心肌细胞凋亡,逆转高血压引起的左室肥厚。
Objective To observe the effrcts of enalapril and valsartan on myocardial apoptosis and the expression of proteins bcl-2/bax in the left ventricle of SHR when the blood pressure was reduced. Methods Eighteen male SHRs were divided into three groups ( n = 6, each group) ,which were treated with enalapril,valsartan ( 30 mg·kg·d^-1) or placebo for 8 weeks. The apoptosis index of cardiomyocytes and expression of protein bcl-2 and bax were detected by using Annexin V/PI and SABC immunohistoehemistry. Results Blood pressure of the three groups at 22 week were enalapril( 126. 0 ± 2.9 )mm Hg, valsartan( 131.7 ±3. 1 )mm Hg, placebo( 218.0 ±4. 1 )mm Hg respectively. The LV/BW ratio was lower:enalapril( 2.72 ± 0. 05 ) mg/g; valsartan( 2. 74±0. 04 )mg/g and placebo( 3.45±0. 06)mg/g. The index of apoptosis in enalapril and valsartan were( 3.98 ± 0. 62) % and (4. 06± 0.44 ) % which was significantly less than that of placebo ( 8.64± 0. 58) %. The expression of bax in placebo group was markedly higher than that in enalapril and valsartan group. The ratio of bel-2/bax in placebo was obviously lower than that of enalaril and valsartan. Conclusions Both enalapfil and valsartan can inhabit myocardial apoptosis through increasing Bel-2/bax to reverse left ventricle hypertrophy in SHR.
出处
《国外医学(内科学分册)》
2006年第10期456-458,共3页
Foreign Medical Sciences(Section of Internal Medicine)
关键词
依那普利
缬沙坦
BEL-2
细胞凋亡
心肌肥厚
Enalapril
Valsartan
Bcl-2
apoptosis
Annexin V/PI
myocardial hypertrophy