摘要
采用反相高效液相色谱法测定了5种植物钩藤中钩藤碱含量。色谱柱为D iamonsil C18;流动相为V(甲醇)∶V(体积分数为0.02%三乙胺溶液)=70∶30混合液;检测波长为250 nm。测定结果表明,钩藤碱的平均回收率为98.98%,RSD=1.31%(n=5)。
An RP-HPLC method was used to determine the rhynchophylline content in five kinds of uncaria. The mobile phase was a mixture of V( CH3 OH) : V( 0. 02% ( volume fraction) C2H5 ) 3 N) = 70: 30, detection wavelength 250 nm, and the average recovery of rhynchophylline was 98.98% , RSD = 1.31% ( n =5). The methods is simple and accurate.
出处
《应用化学》
CAS
CSCD
北大核心
2006年第9期1061-1062,共2页
Chinese Journal of Applied Chemistry
基金
国家中医药管理局资助项目(国中医药科00-01K(ZL))