摘要
用荧光光谱法、紫外分光光度法研究了甲醇与水的体积比为1∶9溶液中恩诺沙星(EFLX)与牛血清白蛋白(BSA)的相互结合作用。研究表明,二者的结合常数为4.16×105L/mol,以其摩尔比为1∶1结合,给体(BSA)与受体(EFLX)间的距离r=4.40 nm。EFLX与BSA的相互结合作用为荧光静态猝灭过程。
The binding reaction between enrofloxacin (EFLX) and bovine serum albumin (BSA) in an aqueous solution of methanol was studied by means of fluorescence and UV-Vis. The results indicate that the association constant(KA) is 4. 16 ×10^5 L/mol, and they reacted in a molar ratio of 1:1 with a binding distance r = 4.40 nm. The Stem-Volmer curve illustrates that the combination reaction of EFLX and BSA is a static quenching process.
出处
《应用化学》
CAS
CSCD
北大核心
2006年第9期957-960,共4页
Chinese Journal of Applied Chemistry
基金
黑龙江省自然科学基金项目(B0202
E200530)
关键词
恩诺沙星
牛血清白蛋白
荧光猝灭
enrofloxacin ,bovine serum albumin ,fluorescence quenching