多环芳烃长期污染土壤的微生物强化修复初步研究

Enhanced Microbial Remediation of Long-term Polycyclic Aromatic Hydrocarbons (PAHs) Polluted Soils

本研究通过室内模拟试验,以急性毒性较强的菲(Phe)和遗传毒性较强的苯并[a]芘(B[a]P)为代表性多环芳烃(PAHs)污染物,以不同C源、通气状况和水分条件为调控因子,对PAHs长期污染土壤的土著微生物强化修复进行初步研究。结果表明,搅动处理使污染土壤中Phe和B[a]P的降解率分别达59.44%和26.14%,而淹水处理使两者降解率分别达46.48%和13.27%。添加C源(淀粉和葡萄糖)处理提高了土壤中PAHs的降解率,且随着C源的施用量而增加。同时也发现污染土壤中PAHs降解菌和微生物总量呈正相关,并随着PAHs降解菌数量的增加,土壤中PAHs降解率也随之提高。可见,土壤中PAHs降解速率主要决定于PAHs的降解菌数量。

An incubation experiment was carried out with farmland soils long-term polluted by PAHs under different carbon sources, aeration condition, and soil moisture contents. The results showed that aeration treatment enhanced degradation rates of Phe and B[a]P in soils to 59.44% and 26.14%, while submerging treatment enhanced them to 46.48% and 13.27%, respectively. Glucose and starch carbon sources were beneficial to the growth of soil microorganisms, thus accelerated the degradation of PAHs in polluted soils. Soil PAHs degradation rate was not only correlated with carbon sources, but also closely correlated with their application contents. The results also indicated soil PAHs-degrading microorganisms were positively correlated with total microorganism numbers in long-term PAHs polluted soils.