期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

重组人胰高糖素样多肽-1(7-36)对胰岛β细胞分泌功能影响的实验研究

Experimental study on the effects (7-36) of recombined human glucagon like peptide-1 on the function of β cells
下载PDF
导出
摘要 目的观察重组人胰高糖素样多肽-1(7-36)(促胰岛素生成药)对大鼠血糖和胰岛β细胞功能的影响。方法12周龄OLETF大鼠随机分为给药组和模型组(n=10),并以同种系非糖尿病LETO大鼠作为对照组;干预2,8周后,处死;于12,14及20周龄时,行口服葡萄糖耐量试验,测定血糖及血清胰岛素。结果经该药治疗,能降低2型糖尿病OLETF大鼠的进食量和体质量,增加血清胰岛素水平,部分改善葡萄糖耐量。结论重组人胰高糖素样多肽-1(7-36)能够改善OLETF大鼠分泌胰岛素的功能,降低血糖。 Objective To investigate the insulinotropic effects of recombined human glucagon like peptide - 1 （7 - 36 ） [ rhGLP - 1 （7 - 36 ） ] on type 2 diabetic rats. Methods Thirty rats were classified into three groups：20 spontaneous type 2 diabetes OLETF rat were randomly divided into trial and model groups, sc, rhGLP - 1 from twelfth week,56 μg·kg^-1 ,once daily and LETO rats as control. At 14th and 20th week, standard oral glucose tolerance and insulin release test were measured respectively. At 14th week, random selected 3 rats from each group were killed, and rests of rats were killed until 20th week. Results rhGLP - 1 treatment caused inhibition of food intake, and reduced body weight of OLETF rats. Subcutaneous administration of rhGLP - 1 increased insulin secretion of type 2 diabetic model, improved glucose tolerance. Conclusion The beneficial effects of rhGLP - 1 in OLETF rats is mediated by an improvement of β cell function.
作者 王昱 郭晓蕙 姜崴
机构地区 北京大学第一医院内分泌科
出处 《中国临床药理学杂志》 CAS CSCD 北大核心 2006年第4期280-283,共4页 The Chinese Journal of Clinical Pharmacology
关键词 重组人胰高糖素样多肽-1(7-36) 糖尿病 胰岛素 recombined human glucagon like peptide - 1 （7 - 36 ） diabetes insulin
分类号 R587.1 [医药卫生—内分泌] R977.15 [医药卫生—药品]
  • 相关文献

参考文献12

  • 1Perfetti R,Merkel P.Glucagon-like peptide-1:a major regulator of pancreatic β-cell function[J].Eur J ofEndocrinol,2000; 143:717-725.
  • 2巩秋红,娄晋宁,叶丽亚,李光伟.重组人胰高血糖素样肽1(7-36)促进INS-1细胞胰岛素释放与合成[J].中华内分泌代谢杂志,2004,20(6):559-560. 被引量：11
  • 3乐嘉静,李湛君,邱财荣,宫泽辉,徐康森.重组人胰高血糖素类多肽-1(7-36)对正常动物降糖作用的实验研究[J].中国临床药理学与治疗学,2004,9(5):527-531. 被引量：7
  • 4乐嘉静,李湛君,邱财荣,宫泽辉,徐康森.重组人胰高血糖素类多肽-1(7-36)对化学致糖尿病模型动物的降血糖作用[J].中国药理学通报,2004,20(10):1171-1173. 被引量：10
  • 5Guerre-Millo M,Rouault C,Poulain P,et al.PPAR-a-null mice are protected from high-fat diet-induced insulin resistance[J].Diabetes,2001 ;50:2809-2814.
  • 6Kawano K,Hirashima T,Mori S,et al.OLETF (Otsuka long-Evans Tokushima fatty) rat:a new NIDDM rat strain[J].Diabetes Res Clin Pract,1994 ;24(Suppl):S317.
  • 7Kawano K,Hirashima T,Mori S,et al.Spontaneous long-term hyperglycemic rat with diabetic complications,otsuka long-evans tokushima fatty (OLETF) strain[J].Diabetes,1992;41:1422-1428.
  • 8Rodriquez de Fonseca F,Navarro M,Alvarez E,et al.Peripheral versus central effects of glucagons like peptide-1 receptor agonists on satiety and body weight loss in Zucker obese rats[J].Metab Clin Exp,2000;49:709-717.
  • 9Holz GG,Kuhtreiber WM,Habener JF.Pancreatic beta-cells are rendered glucose-competent by the insulinotropic hormone glucagonlike peptide-1 (7-37)[J].Nature,1993 ;361:362-365.
  • 10Vilsboll T,Krarup T,Madsbad S,et al.No reactive hypoglycemia in type 2 diabetic patients after subcutaneous administration of GLP-1 and intravenous glucose[J].Diabet Med,2001; 18:144-149.

二级参考文献17

  • 1[1]Brubaker PL. Regulation of intestinal proglucagon-derived peptide secretion by intestinal regulatory peptides[J]. Endocrinology,1991;128(2):3175-28
  • 2[2]Reberge JN, Brubaker PL. Regulation of intestinal proglucagon-derived peptide secretion by glucose-dependent insulinotropic peptide in a novel enteroendocrine loop [J]. Endocrinology,1993;133(1):233-40
  • 3[3]Orskov C, Holst JJ, Knuhtesen S. Glucagon-like peptide GLP-1 and GLP-2,predicted products of the glucagons gene,aresecreted separately from pig small intestine but not pancreas[J].Endocrinology,1986;119(1):1467
  • 4[4]Fehmann HC, Habener JF. Insulinotropic hormone glucagons-like peptide-1(7-37)stimulation of proinsulin gene expression and proinsulin biosynthesis in insulinom a β TC-1 cells[J].Endocrinol,1992;130(3):159-66
  • 5[5]Mojsov S, Weir GC, Habener JF. Insulinotropin: glucagons-like peptide 1 (7-37) co encoded in the glucagons gene is apotent stimulator of insulin release in the perfused rat panicreas[J]. J Clin Invest,1987;79(2):616
  • 6Turton MD et al.Glucagon-like peptide-1 (7-36)-amide confers glucose sensitivity to previously glucose-incompetent beta-cells in diabetic rats: in vivo and in vitro studies[J].J Endocrinol,1997,155(2):369-76.
  • 7Fehmann HC, et al. Insulinotropic horm one glucagons-like peptide-1 (7-36) stimulation of proinsulin gene expression and proinsulin biosynthesis in insulinom a βTC-1 cells[J]. J Endocrinol, 1992,82(3):130-59.
  • 8Holz GG et al. Pancreatic beta-cells are rendered glucose competent by the insulinotropic hormone glucagons-like peptide-1(7-37)[J]. Nature, 1993,56(5):361-2.
  • 9Xu G, Stoffers DA, Habener JF et al. Exendirr4 stimulates both beta-cell replication and neogensis, resulting in increased beta-cell mass and improved glucose tolerance in diabetic rats[J]. Diabetes, 1999,48(2):2270-6.
  • 10Perfetti R, Merkel P. Glucagon-like peptide-1: a major regulator of pancreatic β-cell function. Eur J Endocrinol, 2000,143:717-725.

共引文献20

中国临床药理学杂志
2006年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部