氚－胸腺嘧啶脱氧核苷标记术在判断食管上皮癌变过程中的应用

Evaluation of ￣(3)H- TdR Labelling for procursor Lesions of Human Esophageal Cancer

本文总结了４次（１９８６～１９９０年）在食管癌高发区河南省辉县市孟庄镇，进行的食管癌前病变研究中，通过胃镜普查，将其中３７８例食管上皮活检标本进行３Ｈ—ＴｄＲ标记。结果表明，正常食管上皮１１６例，基底细胞增生２２１例，间变２２例，早期癌１９例，其平均标记指数随着病变的加重而递增。正常与不同病变之间差异有极显著性（Ｐ＜０．００５）；吸烟、年龄及早期癌患者激光治疗前后的标记指数，其差异有极显著性（Ｐ＜０．００５）。采用３Ｈ—ＨｄＲ标记技术显示食管上皮细胞，在肿瘤的多阶段发展过程中的变化，它与基因突变、扩增及染色体结构重组与缺乏等比较，是最容易检测的指标，较单纯组织学分析更客观地反映癌变的发展过程，可作为癌前病变干预试验、治疗的一种可靠的指标。

This paper summarizes endoscopic biopies in 378 subjectes by mean of 3 H-TdR labelling,whose materials were collected form Huizian,Henan province,a hihg risk area for esophageal cancer during 1986-1990 years.The results shoWed that the coverage labelling index of TdR increased progressively in normal subject(116 cases),hasal cell hyperplasia (221 cases),dysplasia(22 cases) and carcinoma in situ(19 cases) of esophageal ephitheliurn(P<0.005).There was remancable difference between normal and abnormal lesions,between smoking and age groups and between pre-and PO8t-treatmen with Nd; YAG.To determine this technical stability and reproducibility,we calculated the results seperately according to different time of invastigation.Application of TdR labelling to represent the proliferation Of esophageal epithelium is more Objective than histology and could serve as a reliable intermedia endpoint biomarker in preventive intervention studies.