大鼠血气屏障细胞成分中吞饮小泡数量及分布差异的定量观察

The Number and Distribution Differences of the Pinocytotic Vesicles in the Cellular Components of the Air-bloodBarrier of Rat

应用计算机辅助的立体计量技术,对正常大鼠肺泡Ⅰ型上皮细胞及毛细血管内皮细胞内的吞饮小泡进行了定量研究。结果如下:(1)单位体积细胞质中的吞饮小泡数:内皮细胞为150.5±39.4,间质细胞38.0±19.6,Ⅰ型上皮细胞135.3±54.7(单位:10^(12)个/cm^3)。(2)吞饮小泡的分布:Ⅰ型上皮细胞腔面为0.05±0.03,基底面为9.08±4.37;毛细血管内皮细胞腔面为14.13±2.95,基底面为11.11±3.27(单位:10~8个/cm^2)。文中分析了Ⅰ型上皮与内皮细胞间上述参数的差异,并讨论了差异的功能意义。

The pinocytotic vesicles in the cellular components of the air-blood barrier of rats were quantified through microcomputer-assisted stereological techniques. The results are as follows:l) The numerical density of the pinocytotic vesicles in the plasma of the alveolar endothelia, interstitial cells and type I epithelia are 150.5±39.4, 38.0±19.6, 135.3± 54.7 respectively (unit 1012/cm3) , 2) In the type I epithelial cells, the vesicular load for the luminal front [Ns (v, epa) ] and that for the intersitial front CNs (v, epi) 3 are 0.05 ± 0.03 and 9.08 ± 4.37 respectively; in the endothelial cells, the vesicular load for the luminal front [Ns (v, enb) ] and that for the intersitial front [Ns (v, eni) ] are 14.13±2.95 and 11.11 ± 3.27 respectively (unit 108/cm2) .The differences in the number and distribution of vesicles in the endothelia and epithelia and their functional significance are discussed.