低氧条件下豚鼠肺Clara细胞超微结构的观察和定量分析

The Observation and Quantitative Analysis of the Ultrastructure of Clara cell in the Lung of Guinea-pig at the Condition of Hypoxia

本文观察了正常成年豚鼠Clara细胞的超微结构和模拟海拔高度5000m 24h、48h和72h后的改变,并对SER和某些细胞器作了定量分析。正常豚鼠Clara细胞顶部充满大量SER和分泌颗粒,GA较发达,表明该细胞为活跃的分泌细胞。低氧24h后Clara细胞呈现早期应激状态。低氧48h和72h后SER和GA高度囊性扩张,体积密度增加,分泌颗粒数目密度增加,但平均体积在72h组明显下降。这些结果表明低氧导致Clara细胞超微结构改变,功能亢进,分泌增强,提示该细胞可能参与表面活性物质的形成。

By using the transmission electron microscopy, the ultrastructure of the Clara cell of the normal adult guinea-pig and its change under the simulated elevation of 5 000m after 24 h, 28 h and 72 h are studied. Meanwhile the quantitative analysis of the SER and some organelles are carried out. Clara cell of the normal guinea-pig contains abundent SER and secretory granules with a prominent Golgi apparatus. The facts suggest that Clara cell has the secretory characteristics. After 24 h hypox ia, Clara cells appear the early states of response. After 48 h and 72 h the SER and Golgi apparatus appear highly exaggerated. Their volume density is increased and the numeral density of secretory granules also increased. The average volume of the secretory granules decreased apparently in 72 h groups. These results show that the hypoxia cause the ultrastructural changes and enhance the evidence that Clara cells may participate the formation of the surfactant.