摘要
Objective To investigate the relationship between trans, trans-muconic acid （ttMA） as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS （2） （5u） column （Ф4.6 min× 150 mm） and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water （v/v, 1：2：10：87）. The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals. Results A correlation coefficient （r = 0.9963 ） was found for ttMA ranging 0.10-10.00 μg/mL. The limit of detection was 0.10μ g/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y=0.859＋0.108C （before work, r=0.6200） or Y=1.980＋0.179C （after work, r=0.7930）. Conclusion This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.
Objective To investigate the relationship between trans, trans-muconic acid （ttMA） as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS （2） （5u） column （Ф4.6 min× 150 mm） and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water （v/v, 1：2：10：87）. The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals. Results A correlation coefficient （r = 0.9963 ） was found for ttMA ranging 0.10-10.00 μg/mL. The limit of detection was 0.10μ g/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y=0.859＋0.108C （before work, r=0.6200） or Y=1.980＋0.179C （after work, r=0.7930）. Conclusion This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.
