饲料中维生素B_6对皱纹盘鲍幼鲍蛋白质代谢的影响

Effect of dietary vitamin B_6 on the protein metabolism in juvenile abalone Haliotis discus hannai Ino

以皱纹盘鲍(Haliotis discus hannaiIno)幼鲍为研究对象,探讨维生素B6对其蛋白质代谢的影响。设计了3种维生素B6不同含量(0,40,4 000 mg/kg)的半精制饲料,在循环水养殖系统中养殖幼鲍240 d。结果表明,皱纹盘鲍内脏团和肌肉中磷酸吡哆醛(PLP)的含量随饲料中维生素B6的添加而显著升高(P<0.05)。饲料中不同维生素B6水平对肌肉组织中蛋白含量没有显著影响(P>0.05),而显著升高内脏团中蛋白质的含量(P<0.05)。内脏团和肌肉中的谷丙转氨酶(ALT)和谷草转氨酶(AST)活力随着饲料中维生素B6添加量的升高而显著增强,分别在维生素B6的添加量为4 000 mg/kg时取得最大值(P<0.05)。内脏团中所检测的各种氨基酸的含量与饲料中维生素B6添加量没有显著影响(P>0.05)。然而,肌肉中的天冬氨酸(Asp)、苏氨酸(Thr)、丝氨酸(Ser)、牛磺酸(Tau)的含量随着饲料中维生素B6添加量的升高而显著增加,并且分别在维生素B6添加量为4 000 mg/kg时取得最大值(P<0.05)。肌肉中甘氨酸(Gly)、丙氨酸(Ala)、缬氨酸(Val)、蛋氨酸(Met)的含量在两个维生素B6添加组(40 mg/kg和4 000 mg/kg)之间没有显著差异(P>0.05),但均显著高于维生素B6缺乏组(0 mg/kg)的值(P<0.05)。

A 240-day growth experiment was conducted in a re-circulated water system to investigate the effect of dietary vitamin B6 on the protein metabolism of abalone Haliotis discus hannai Ino. Juvenile abalone （initial weight： 0. 834±0. 002 g; initial shell length.. 1. 668±0. 003 cm） was fed to satiation with one of 3 semi-purified diets containing vitamin B60, 40, 4 000 mg/kg. Results showed that the contents of pyridoxal-5- phosphate （PLP） in viscera and muscle of abalone significantly increased with the dietary vitamin B6 supplements （P〈0. 05）. Dietary vitamin B6 significantly increased protein contents in viscera （P〈0. 05）, not in muscle （P〉0.05） of abalone. The activities of alanine aminotransferase（ALT） and aspartate aminotransferase （AST） both in viscera and muscle were significantly enhanced by dietary vitamin B6 （P〈0.05）, and their highest values were found in the treatment with 4000mg/kg dietary vitamin B6 supplement. There were no significant effects of dietary vitamin B6 on the contents of analyzed amino acids in viscera. The contents of Asp, Thr, Ser and Tau in the muscle of abalone were significantly increased by dietary vitamin B6 （P〈0.05）. There were no significant differences in the contents of Gly, Ala, Val,and Met in the muscle of abalone between the two vitamin B6-supplemented treatments （40 mg/kg and 4 000 mg/kg） （P〉0.05）, but were significantly higher than those in the dietary vitamin B6 deficient treatment （0 mg/kg） （P〈0.05）.