HPLC-MS^n法分析复方苦参注射液的化学成分

Analysis of chemical components in Compound Kushen Injection by HPLC-MS^n

目的:明确复方苦参注射液(苦参白土苓)的化学成分,建立含苦参的复方制剂HPLC-MSn鉴定方法。方法:应用HPLC-ESI-MSn方法,其中,液相色谱采用梯度洗脱,质谱用正离子模式检测。结果:从复方苦参注射液中分离鉴定了11种化学成分。结论:本法简便,快速,可用于中药复方化学成分的分析。

AIM： To study the chemical components in Compound Kushen Injection （Radix Sophorae Flavescentis）, and to develop a method to identify the composition contained Radix Sophorae Flavescentis. METHODS： The sample was analysed with gradient elution by HPLC. Agilent MS TRAP equipped with ESI source was used as detector and operated in positive ion mode. RESULTS： 11 components were identified in Compound Kushen Injection. CONCLUSION： This method is simple, rapid and can be used for the analysis of components in Compound Kusen Injection.