摘要
目的：建立RP-HPLC测定紫锥菊提取物中2种咖啡酸衍生物含量的方法，同时测定5种样品。方法：采用RP．HPLC同时测定紫锥菊提取物中2种主要咖啡酸衍生物：单咖啡酰酒石酸、菊苣酸的含量。色谱条件为Agilent ZORBAX Stable Bond C18柱（5μm，4．6mm×250mm）；流动相：A（乙腈）-B（0．1％磷酸溶液）梯度洗脱，流速：1．2mL／min；检测波长：330nm。结果：2种主要咖啡酸衍生物在色谱条件下有良好的分离度，浓度与峰面积之间线性关系良好，线性范围：单咖啡酰酒石酸在0．064～0．416μg，菊苣酸0．1—0．7μg。平均回收率：单咖啡酰酒石酸为99．37％．RSD为1．50％；菊苣酸为100．44％，RSD为1．71％。结论：方法简便、精确、专属性强，可作为提取物和研发新药的质量控制。
AIM： To estahlish a RP-HPLC method of determining two caffeic acid derivatives in Echinacea extract and to determine 5 Echinacea purpurea root extracts. METHODS： RP-HPLC was applied to determine 2 caffeic acid derivatives in E. purrpurea extract：caftaric acid and chicoric acid. HPLC conditions were as follows： Agilent ZORBAX StableBond C18 column （5 μm,4.6 mm×250 mm） was used , with A（acetonitrile）-B（0.1% H3POa ） gradient elution as a mobile phase. The flow rate was 1.2 mL/min. The detection wavelenth was set at 330 nm. RESULTS： 2 caffeic acid derivatives were separated well. The linear range for cafiaric acid and chicoric acid were 0. 064-0. 416μg and 0. 1-0.7μg, respectively. Its average recoveries were 99.37% with RSD of 1.50% and 100.44% with RSD of 1.71%, respectively. CONCLUSION： The method is simple, accurate,strong specificity and can be used to control the quality of E. purpurea extract and new medicine development.
出处
《中成药》
CAS
CSCD
北大核心
2006年第10期1493-1497,共5页
Chinese Traditional Patent Medicine
