金雀异黄素对IL-1α刺激破骨样细胞的组织蛋白酶K表达的影响(英文)

Effects of genistein on cathepsin K expression stimulated by inter-leukin-1αin osteoclast-like cells

目的:探讨金雀异黄素对白细胞介素1α(IL-1α)刺激破骨样细胞组织蛋白酶K(CK)表达的作用。方法:从人骨巨细胞瘤组织中纯化出破骨样细胞(OCLs),用不同浓度的金雀异黄素或17β-雌二醇(17β-E2)孵育,并设空白对照组和阳性对照组,采用RT-PCR和Western blot方法,观察IL-1α刺激后CK的表达。结果:与空白对照组相比,IL-1α刺激CK表达显著增加(P<0.01);金雀异黄素在转录水平下调IL-1α刺激后CK的表达,且呈剂量依赖关系(r=0.68,P<0.01);金雀异黄素下调IL-1α刺激后CK的蛋白表达,且呈剂量依赖关系(r=0.61,P<0.01)。雌激素受体拮抗剂ICI 182.780可以部分抑制金雀异黄素的上述作用。结论:金雀异黄素可通过破骨样细胞的雌激素受体部分抑制IL-1α刺激后CK的表达。

AIM： To discuss the effects of phytoestrogenic-genistein on cathepsin K （CK） expression stimulated by interleukin-1α （IL-1α） in osteoclast-like cells （OCLs） . METHODS： The OCLs were isolated from tissue of human giant cell tumor of bone （GCT）. The cells treated with reagents were divided into 7 groups including control （treated with phenol red-free-DMEM）, vehicle （treated with 1.2 nmol·L^-1 IL-1α）, 10^-10 - 10^-6 genistein, genistein＋ ICI 182.780, and 17β-estrodiol （17β-E2） group. The cells were treated with 1.2 nmol·L^-1 IL-1α after pre-treated with genistein or 17β-E2 for 48 h （excluded the control group） . Expression of CK was determined by reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blot in OCLs stimulated by IL-1α in the presence of genistein or 17β-E2. RESULTS： The obvious increase of expression of CK by IL-1α in vehicle group was noted in comparing with control group （P 〈 0.01） . Genistein down-regulated CK gene expression stimulated by IL-1α at the transcription level in a dose-dependent manner （r = 0.68, P 〈 0.01） . Genistein down-regulated CK protein expression stimulated by IL-1α also in a dose-dependent manner （r = 0.61, P 〈 0.01 ）. The effects of genistein were abrogated partly after treatment with the estrogen receptor antagonist ICI 182.780. CONCLUSION： Genistein inhibits CK expression stimulated by IL-1α partly through estrogen receptor in OCLs.