山东省HIV-1流行株多基因区亚型分析

Subtyping analysis of HIV-1 strains in Shandong province

目的了解流行于山东省境内HIV-1毒株的亚型,尤其是流行重组型毒株的种类及变异情况,分析其来源并推测其流行趋势。方法采集93份HIV-1感染者的外周静脉防凝血,提取前病毒DNA进行体外扩增,获得包膜蛋白(env)、核心蛋白(gag)、tat区基因的核酸片段,并对各基因区核苷酸进行测定和分析。结果发现在所有目标人群中共存在B、B′、C3种亚型以及CRF07-BC、CRF08-BC、CRF01-AG和CRF01-AE4种重组毒株,其中B′亚型数量最大71株(占77.2%),CRF01-AE9株(占9.8%),CRF07-BC、CRF0B-BC各3株(均占3.3%),B、C和CRF02-AG各2株(占2.2%)。3个基因区env区变异最大,gag和tat相对保守。3种优势亚型中,A/E变异最大,B'次之,B'/C最保守。结论山东省HIV-1流行毒株亚型众多,各优势毒株发生了不同程度的变异,在山东省有流行加快的趋势,应加强防控。

Objective To understand the distribution of HIV-1 strain subtypes and their variation in Shandong province, analyse their source and predict their epidemic trend. Methods 93 DNA fragments of HIV-1 env, gag, tat gene were amplified by nested PCR from those infected with HIV-1, in 2002-2003. Their C2-V3, P17/ P24, 1st exon of tat and adjacent region were sequenced. Results Sequence analysis showed that there were 7 HIV- 1 or circulating recombinant forms （CRFs）, B＇ （n = 71 ）, CRF01-AE （n = 9 ）, CRF07-BC （n = 3）, CRFOB-BC （n=3）, B（n=2）,C（n=2）, CRF02-AG（n=2）. B＇ strains＇ variation were much more than CRF01-AE and B＇/C. Conclusion There were many kinds of subtype and CRFs, and their genomes generated obvious variation. Therfore, the quickening trend of HIV-1 infection urged the strengthening of HIV control in Shandong province.