中药复方对肝纤维化小鼠白细胞介素-2和一氧化氮的影响

Influence of complex prescription of Chinese materia medica on interleukin 2 and nitrogen monoxidum of mice with hepatic fibrosis

目的研究中药复方防治肝纤维化小鼠的免疫调节作用。方法将小鼠分成不同组别,采用不同剂量的二参泽术汤和丹参桃芎汤喂养20 d后取血,用ELISA法测定白细胞介素-2(IL-2);用硝酸还原法测一氧化氮(NO)。结果不同剂量的二参泽术汤和丹参桃芎汤组IL-2、NO值明显高于对照组(P均<0.001)。结论二参泽术汤和丹参桃芎汤对小鼠的免疫调节具有积极作用,为防治肝纤维化过程中中药扶正提供理论依据。

Objective It is to study the immunoloregulation action of complex prescription of Chinese materia medica preventing and curing hepatic fibrosis. Methods The mice were divided into different groups and were taken blood after they had been fed different dose of Ershenzezhu soup and Danshentaoxiong soup for 20 days. Interleukin 2 （IL -2） was determined with ELISA method and nitrogen monoxidum （NO） was determined with nitric acid reduction method. Results The values of IL - 2 and NO in different dose groups of Ershenzezhu soup and Danshentaoxiong soup were obviously higher than those in control group （P 〈0.001 ）. Conclusion Ershenzezhu soup and Danshentaoxiong soup have energetic effect on immunoloregulation of mouse, which provides theory basis for supporting the healthy energy of Chinese materia medica in the process of preventing and curing hepatic fibrosis.