用抗BMP单克隆抗体分析骨形成蛋白在骨和牙胚中的分布

Analysis of the Distribution of BMP in Bone and Tooth Germs by Monoclonal Antibody Against Bone Morphogenetic Protein

作者用杂交瘤技术获得了纯系细胞株,能稳定分泌抗BMP单克隆抗体。该抗体能与人的骨和牙胚发生反应,具有很高的特异性。免疫组化染色结果表明,BMP存在于骨基质的胶原纤维、新生的骨基质、骨母细胞和骨髓中的未分化间充质细胞中;在人的牙胚中,BMP主要分布在前期牙木质、外釉细胞和造釉细胞以及形成牙槽骨的牙囊细胞中,表明骨的生长和形成以及牙齿的发育都与BMR密切相关。bBMP-McAb能与人的骨和牙胚发生反应,证实人和牛骨的BMP的抗原决定簇是相同的。BMP活性阻断实验证明,新研制的单克隆抗体能有效地封闭BMP活性基因。利用bBMP McAb这一特点,不仅可以纯化BMP,而且可以用来抑制骨的生长,为临床治疗骨肉瘤和其它骨疾病开拓了广阔的前景。

A cell strain steadily secreting monoclonal antibody against BMP was obtained by hybridoma technique. The monoclonal antibody specifically reacts with the cells of human bone and tooth germs on paraffin embedded tissue sections. Immu-nohistochemical staining shows that BMP is distributed in collagen fibres in normal bone, new osteoid tissue, osteoblasts and the cells of bone icarrow. BMP may also be found in human tooth germs, predentin, the cells of outer and inner enamel epithelium, and the cells of dental sac generating alveolar bone. The results prove that antigenic determinants are the same in both the human and bovine BMP. BMP active inhibition test suggests that tins antibody may block the function group of BMP. The ability of the monoclonal antibody to detect antigen and inhibit generation of new bone ma 'kes it poten tially useful in purification of BMP and treatment of os teosarcoma and other bone diseases.