骨髓间充质干细胞通过参与血管形成改善心脏的功能

Mesenchymal stem cells Involved in vascularization,and improve heart function in rat acute myocardial infarction model.

目的探索心肌梗死区域移植的骨髓间充质干细胞(MSC)通过参与血管形成,增加血管数目,改善心肌梗死及周边区域的血供,对损伤心脏功能的影响。方法采用成年近交系W istar大鼠,经左冠状动脉前降支结扎建立急性心肌梗死模型。术后1周,将来自同种供体、经体外扩增和DAPI标记的MSCs植入梗死区。移植后4周测定心脏功能,随后取材、连续冰冻切片,观察心肌组织形态学的变化以及MSC向血管特异性血管内皮黏附分子(CD31)和血管平滑肌肌动蛋白(SM-Actin)的表达。结果植入梗死区中的MSC表达CD31和SM-Actin,且有少数细胞形成血管样结构。移植组血管数目增多,梗死区面积缩小,左心室壁厚度增加,心脏功能改善。结论移植的MSC通过分化为血管平滑肌细胞和血管内皮细胞参与血管形成,改善梗死区的血液供应,达到改善心脏功能的目的。

Objective To investigate improvement of heart function through rat marrow mesenchymal stem cells （MSCs） involved in Vascularization in acute myocardial infarction （AMI）. Methods MSCs were isolated from adult in bred Wistar rats, cultured and expanded in vitro, and labeled with DAPI, The animal model of AMI was established by the left anterodecendant arteries （LAD） occlusion （60min） with reperfusion in rat s of the same species. One week after operation, 1.0×107MSCs were injected into infarcted areas . Four week after transplantation, cardiac func- tion was analyzed. And heart tissues were taken after the measurement of hemodynamics. Histology and morphometric measurement was observed by image Analysis system in microscope and MSCs expressions of CD31 and SM - Actin detected by immunofluorescent cytochemistry. Results The transplanted MSCs in AMI area had significantly higher expression rates of CD31 and SM - Actin 4 weeks after transplantation. There were a decreased infarct size , greater vascular density, thicker left ventricle wall in MSCs-treated group. Conclusion In a rat AMI model, MSCs differentiated into smooth muscle cells and endothelial ceils, resulting in vascularity and improved cardiac function.