阿托伐他汀对实验性变态反应性脑脊髓炎模型脑组织IL-1β、TNF-α影响的研究

Study on Variation of IL-1β and TNF-α of Experimental Allergic Encephalomyelitis' brain with Atorvastatin Therapy.

目的探讨实验性变态反应性脑脊髓炎模型(EAE)脑组织细胞因子的变化,探讨阿托伐他汀对EAE发病保护作用的免疫调节机制。方法皮下注射粗制碱性髓鞘蛋白(MBP)建立EAE模型。用40只豚鼠分成4组:正常对照组、EAE对照组、EAE小剂量组和EAE大剂量组,每组10只,雌雄各半,用放射免疫法测定正常对照组、EAE各组高峰期脑组织细胞因子白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)含量。结果EAE对照组、EAE小剂量组和EAE大剂量组脑组织细胞因子IL-1β水平分别为2.85±0.70、1.97±0.44、1.57±0.45 ng/mg,显著高于正常对照组0.51±0.02 ng/mg,EAE大、小剂量组脑组织IL-1β水平比EAE对照组低,EAE大剂量组脑组织IL-1β水平比EAE小剂量组低(P<0.05);EAE对照组、EAE小剂量组和EAE大剂量组脑组织细胞因子TNF-α水平分别为2.83±0.90、1.98±0.44、1.46±0.35 ng/mg,显著高于正常对照组水平(1.13±0.28 ng/mg),EAE大、小剂量组脑组织TNF-α水平比EAE对照组低;EAE大剂量组TNF-α水平比EAE低剂量组低(P<0.05)。结论EAE豚鼠存在明显免疫功能紊乱,阿托伐他汀有降低EAE模型脑组织IL-1β、TNF-α水平作用,其对EAE发病的免疫保护机制可能是通过抑制IL-1β、TNF-α产生而发挥。

Objective To explore the cellular immune of experimental allergic encephalomyelitis in Guinea pig and the immunol regulating mechanisms of Atorvastatin. Methods Experimental allergic encephalomyelitis in Guinea pig was induced by subcutaneous administrating coarse myelin basic protein. 40 Guinea pigs were assigned to four groups ： the normal group , the EAE control group, Low dose treating EAE group , high dose treating EAE group. The levels of cytokines （IL-1β, TNF-α） in brain at fastigium were analyzed by radioimmunoassay. Results The levels of IL-1βand TNF -αin the EAE groups＇brain at fastigium were higher than those in the normal group＇. Comparing the low dose treating EAE group or high dose treating EAE group with the EAE control group, the levels of IL-1βand TNF-αwere decreased. Conclusion The immunol treatment role of Atorvastatin on EAE may he played by inhibiting production of IL-1βand TNF-α.