温度和pH敏感性壳聚糖接枝物对辅酶A的控制释放

Temperature and pH Sensitivity of Acrylic Acid and Poly(N-isopropylamide) Graft Chitosan Polymer and Its Controlled Delivery on Coenzyme A

利用接枝共聚合成了具有聚丙烯酸（PAA）和聚异丙基丙烯酰胺（PNIPAm）支链的壳聚糖（CS）聚合物[P（CS—g—AA／PNIPAm）]，研究了其水溶液的温度和pH敏感性，并利用其性能探讨了P（CS·g—AA／PNIPAm）对辅酶A的控制释放。紫外光谱分析结果表明，P（CS—g—AA／PNIPAm）水溶液比异丙基丙烯酰胺均聚物的最低临界溶解温度（LCST）略高。在pH：9、温度为24—50℃或温度为24℃、pH=3．7—9的条件下，聚合物都能对辅酶A（CoA）进行控制释放。对其控制释放机理进行初步探讨。

A kind of temperature and pH-sensitive polymer P （CS-g-AA/PNIPAm） was prepared by graft and copolymerization of chitosan （CS） , acrylic acid （ AA ） and N-isopropylamide （ NIPAm ）. UV analysis showed that the low critical solution temperature （LCST） of P（CS-g-AA/PNIPAm） is higher than that of poly （N-isopropylamide） in aqueous solution. At pH = 9 and temperature range of 24 - 50 ℃ ,or at 24 ℃ and pH between 3.7 and 9, P （CS-g-AA/PNIPAm） can well control the delivery of coenzyme A and the release mechanism of coenzyme A was also investigated.