用单克隆抗体检测体液中新的胃癌相关抗原

Determination of MG-Ags in Body Fluids and Their Diagnostic Value in Tumor Patients

作者对一组新的胃癌相关抗原 MG-Ags(MG5-Ag,MG7-Ag,MGd1-Ag,MG9-Ag)用单克隆抗体免疫酶ELISA微球法进行体液检测,以探讨其在肿瘤诊断中的意义。共检测胸水95例(其中肺癌性胸水45例),腹水51例(其中各种癌性腹水33例),胃液74例〔其中胃癌22例)。分别以良性疾病胸水,腹水和胃液MG-Ags检测吸收率的均数加3个标准差作为判断肿瘤的阳性阈值。MG-Ags检测的阳性率分别是:肺癌胸水73.3％;胃癌、肠癌腹水83.3％;胃癌胃液为63.6％;而卵巢癌、肝癌腹水则为阴性。在良性疾病中MG-Ags检出的假阳性率分别为:胸水6％;腹水5.5％;胃液5.8％与良性疾病组时照MG-Ags检测对肿瘤诊断的总符合率为85.8％实验结果提示体液中MG-Ags的检测对肺癌、胃癌及肠癌具有较高的诊断价值。

A new group of gastric cancer associated antigens (MG-Ags) in ascitic fluid, gastric juice and pleural effusion of patients with tumors were detected by F.LISA method (micro-bead method) with the monoclonal antibodies against gastric cancer.The average absorbance values of control group plus 3 standard deviations were arbitrarily set as their positive thresholds respectively. The positive rate were 73. 3%( 33/45) in pleural effusion of patients with lung cancer, 83. 3%( 20/24) in ascitic fluid of patients with gastrointestinal cancer, 63. 6%( 14/22) in gastric juice of patients with gastric cancer. They were not found in ascitic fluid of patients with liver cancer or ovarian cancer. The positive rate were only found to be 6%( 3/50) in pleural effusion, 5. 5%( 1/18) in ascitic fluid and5. 8%(3/52) in gastric juice of patients with benign diseases. The results of these studies suggested that determination of MG-Ags in ascitic fluid, pleural effusion and gastric juice of patients can play an important role in the diagnosis of gastrointestinal cancer and lung cancer.