摘要
AIM: To explore the anti-hepatitis B virus (HBV) effects of Boehmeria nivea (B. nivea) root extract (BNE) by using the HepG2 2.2.15 cell model system. METHODS: Hepatitis B surface antigen (HBsAg), hepatitis B virus e antigen (HBeAg), and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS: In HepG2 2.2.15 cells, HBeAg (60%, P < 0.01) and particle-associated HBV DNA (> 99%, P < 0.01) secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION: BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.
AIM： To explore the anti-hepatitis B virus （HBV） effects of Boehmeria nivea （B. nivea） root extract （BNE） by using the HepG2 2.2.15 cell model system. METHODS： Hepatitis B surface antigen （HBsAg）, hepatitis B virus e antigen （HBeAg）, and HBV DNA were measured by using ELISA and real-time PCR, respectively. Viral DNA replication and RNA expression were determined by using Southern and Northern blot, respectively. RESULTS： In HepG2 2.2.15 cells, HBeAg （60%, P 〈 0.01） and particle-associated HBV DNA （〉 99%, P 〈 0.01） secretion into supernatant were significantly inhibited by BNE at a dose of 100 mg/L, whereas the HBsAg was not inhibited. With different doses of BNE, the reduced HBeAg was correlated with the inhibition of HBV DNA. The anti-HBV effect of BNE was not caused by its cytotoxicity to cells or inhibition of viral DNA replication and RNA expression. CONCLUSION： BNE could effectively reduce the HBV production and its anti-HBV machinery might differ from the nucleoside analogues.
基金
DCB-094EN203 and NSC 93-2311-B-007-002
关键词
乙型肝炎
肝炎病毒
病毒感染
治疗
Boehmeria nivea
Medicinal herb
Antiviral agent
Hepatitis B virus
Anti-hepatitis B virus
HepG2 2.2.15
