恶性间皮瘤可能与致瘤性猿猴病毒SV40无关

Relationship between the malignant mesothelioma and simian virus 40 in China:a study of 17 cases

目的探讨致瘤性猿猴病毒 SV40(simian virus 40)是否与中国人恶性间皮瘤的发生相关。方法从蜡块中提取17例恶性间皮瘤组织中的 DNA 后,用三组引物对 SV40大 T 抗原(TAg)的基因片段分别进行聚合酶链反应(PCR)扩增,另外,用两种 SV40相关抗体(Pab101和 Ab-2)分别进行免疫组织化学染色,检测肿瘤组织中是否存在 SV40 TAg。结果 (1)一组引物的 PCR 反应仅有3例扩增出了 SV40 TAg 的基因片段,其余两组引物的 PCR 反应均为阴性。(2)两种抗体的免疫组织化学染色均未检测出 SV40 TAg。结论中国人恶性间皮瘤与 SV40感染的关系可能不密切。

Objective To investigate whether simian virus 40 （SV40） was related to patients of malignant mesothelioma in China. Methods Paraffin-embeded samples of 17 patients with malignant mesothelioma were collected. After isolation of DNA from paraffin blocks, polymerase chain reaction （PCR） analyses were performed using three different sets of primer for detection of SV40 large T antigen gene. These samples were also immunohistochemically evaluated for expression of SV40 TAg protein with two different anti-SV40 Tag （Pab101 and Ab-2 ）. Results Only one of the three primer pairs successfully amplified SV40 genome in three malignant mesothelioma samples. No immunopositive staining for SV40 TAg was found in any of the samples. Conclusions The study shows that malignant mesothelioma in China may be independent of SV40 infection.