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早期生长反应基因1在急性胰腺炎肝脏损害机制中的作用 被引量：3

The role of Egr-1 in liver injury in acute pancreatitis in rat
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摘要 目的研究早期生长反应基因1(Egr-1)在急性胰腺炎肝脏损害机制中的作用。方法24只雄性Wistar大鼠随机分为4组,分别用生理盐水,1%、3%及5%牛磺胆酸钠溶液进行相应处理,观察各组大鼠肝组织Egr-1免疫组化染色结果并进行胰腺病理评分,检测血清AST、LDH及TNF-α和IL-1β水平。从正常Wistar大鼠分离培养肝细胞,观察原代培养肝细胞在TNF-α刺激及PD98059预处理后Egr-1mRNA表达情况。结果肝组织Egr-1表达与急性胰腺炎肝损害程度呈正相关,且原代培养肝细胞Egr-1mRNA表达水平与肝细胞损害程度呈正相关。结论Egr-1可能参与急性胰腺炎肝损害过程,并且其作用机制部分依赖于细胞外信号调节激酶1/2(ERK1/2)。 Objective To evaluate the role of early growth response genel （Egr-1） in liver injury in taurocholate-induced acute pancreatitis rat model. Methods Twenty-four male rats were divided into 4 groups. Egr-1 immunohistochemistry staining and pancreatic pathologic scoring were assessed, and the serum levels of AST, LDH, TNF-a and II-1 β were measured. Egr-1 mRNA levels of primary hepatocyte culture stimulated with TNF-a and pretreated primary hepatocytes with PD98059 followed by TNF-a stimulation were also observed. Results Egr-1 expression of liver correlated with degree of liver injury in acute pancreatitis in rat, and high Egr-1 mRNA expression of primary hepatocyte was observed when hepatocyte injury occurred. After pretreated with PEN8059, TNF-a-stimulated hepatocytes showed a lower level of Egr-1 mRNA compared with hepatocytes without pretreatment. Conclusions Egr-1 may play an important role in liver injury in acute pancreatitis, and this effect depended partly upon extracellular signal-regulated kinase 1/2 （ERK1/2） pathway.
作者 邹树 田伏洲 崔建峰
机构地区 成都军区总医院全军普通外科中心
出处 《解放军医学杂志》 CAS CSCD 北大核心 2006年第10期979-982,共4页 Medical Journal of Chinese People's Liberation Army
关键词 早期生长反应基因1 胰腺炎 肝损害 有丝分裂素激活蛋白激酶类 early growth response genel pancreatitis liver injury mitoger activated protein kinases
分类号 R657.51 [医药卫生—外科学]
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参考文献8

  • 1孔雷,韩天权,汤耀卿,张圣道.急性胰腺炎发病机制研究进展[J].中华肝胆外科杂志,2004,10(12):853-855. 被引量：23
  • 2Guha M,O'Connell MA,Pawlinski R et al.Lipopolysaccharide activation of the MEK-ERK1/2 pathway in human monocytic cells mediates tissue factor and tumor necrosis factor alpha expression by inducing Elk-1 phosphorylation and Egr-1 expression.Blood,2001,98(5):1429
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  • 6陈学清,姜泊,宋于刚,智发朝,巩兰波.早期生长反应因子-1和组织因子在大鼠胰腺炎组织中的表达[J].第一军医大学学报,2004,24(11):1245-1247. 被引量：13
  • 7Yao J,Mackman N,Edgington TS et al.Lipopolysaccharide induction of the tumor necrosis factor-alpha promoter in human monocytic cells.Regulation by Egr-1,c-Jun,and NF-kappaB transcription factors.J Biol Chem,1997,272(28):17795
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二级参考文献20

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解放军医学杂志
2006年 第10期

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