利用新开发的SSR标记分析花生栽培种的多态性

Analysis of DNA Polymorphism in Peanut Cultivars Using New Microsatellite Markers

采用12份花生栽培种材料,对通过建库、筛库、杂交富集的策略开发设计出来123对SSR引物进行评估,并基于品种间简单匹配系数进行聚类分析。研究表明有44对SSR引物能产生多态性片段,总共获得176条带,其中107条(60.8%)为多态性带。平均每对引物产生4条带,2.43条为多态性带,多态性条带的比率为11.1%～100%,PIC值为0.245～0.886,平均为0.677;根据聚类结果显示,在简单匹配系数值为0.85处,根据SSR带型12个花生品种可分成3个品种群(Ⅰ、Ⅱ、Ⅲ)。研究结果表明,新开发的44对SSR引物在分析花生栽培种DNA多态性方面非常有用。

SSR primers published for peanut, along with those from a strategy involving hybridization enrichment, library construction were evaluated by using 12 peanut cultivars mainly from Shandong. Cluster analysis＇was carried out based on simple matching coefficients between varieties. The results showed that 44 SSR primer pairs were found to produce 176 bands , and 107 （60.8%）were polymorphic. On an average, each primer pair produced 2.43 polymorphic bands in a total of 4 bands. The polymorphic percentage and PIC ranged from 11.1%-100% and 0.245-0.886, respectively. The average of PIC was 0.677.The result of cluster analysis indicated the majority of 12 accessions could be divided into three groups （ Ⅰ , Ⅱ, Ⅲ） The result showed that microsatellites are very useful DNA marker to analyze DNA polymorphism in cultivated peanut. The high genetic similarity of Shandong peanut at DNA level indicated gene bases, which need broadening in cultivar breeding through utilization of elite exotic germplasm resources.