小麦新鲜根系铁氰化钾还原酶与氢、钾跨膜运输的关系

Relation between Ferricyanide Reductase and Transfer of Proton and Potassium Across Plasma Membrane in Fresh Roots of Wheat

为研究麦根质膜氧化还原反应与钾离子吸收的关系,比较不同基因型小麦吸钾差异,以水培和短期生物吸收试验方法,检测到小麦根系铁氰化钾还原酶的存在,而且不同品种铁氰化钾还原酶还原活性不同。反应液中由于根系内外质子和钾离子的迁移,加与不加铁氰化钾,不同品种根系都引起了溶液pH的改变,但外部电子受体Fe(CN)63-的存在即铁氰化钾还原酶底物的存在加强了溶液pH改变(更多为降低)的程度。铁氰化钾还原也明显影响了根系钾离子的吸收或外排动态,但不同品种影响不同。小麦根细胞膜氧化还原系统明显影响质子和钾离子的跨膜运输,不同品种影响不同。

In order to study the relation between ion absorption and Ferricyanide reductase, we compare the differences of several wheat cuhivars, and hydroponics and short-term uptake tests were undertaken. Ferricyanide reductase was detected in wheat root, and there existed difference in activity of Ferricyanide reductase among wheat cuhivars. Whether Ferricyanide added or not to reaction solution, the roots of all wheat cuhivars could bring about pH change because of the transfer of potassium and proton across plasma 3- membrane of roots. Moreover, the existence of Fe（CN）6^3- as the reaction substrate to Ferricyanide reductase 3- enhanced the degree of pH change. The deoxidization of Fe（CN）6^3- influenced on the uptake/exudation of potassium across plasma membrane of roots, and with different effects on different cuhivars. Ferricyanide reductase have an effect on ion transport across cell membrane,and with different effects on different cuhivars.