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基因佐剂注射时相对HBV preS_2S疫苗免疫作用的影响

Raised immune responses of HBV preS_2S DNA vaccines by administrating genetic adjuvant at different time
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摘要 目的:HBV preS2S基因疫苗接种不同时相应用佐剂pcDNA3·1IL-2/Fc,观察其对诱导免疫反应效果的影响。方法:采用HBV preS2S DNA基因疫苗作为基础免疫,重组质粒pcDNA3·1IL-2/Fc作为佐剂加强免疫,设计实验组与HBVpreS2S基因疫苗同时及在注射BALB/c小鼠后第3天加用佐剂,采用0、2、4周的方案接种,检测各次接种后抗体水平、免疫脾细胞的杀伤活性、增殖活性和细胞因子分泌水平。结果:HBV preS2S注射3天后应用佐剂的免疫小鼠,其抗体滴度、免疫脾细胞杀伤活性、增殖活性和Th1型细胞因子分泌水平均比同时免疫组、单独应用HBV preS2S组及空载体对照组明显增强。结论:预先接种疫苗后加用IL-2/Fc佐剂,能明显增强疫苗免疫效应。 Objective：To study the different effect of fused gene IL-2/Fc administrated at different time on immune responses induced by HBV preS2S genetic vaccine. Methods： BALB/c mice were immunized with purified recombinant plasmlds at 0, 2, 4 weeks. Two groups were designed. One group were injected with pcDNA3.1S2S at first, then pcDNA3. IIL-2/Fc was administrated after three days. While the other group were injected two plasmids at the same time. We compared the immune responses through detecting the anti-HBs titers, CTL cytotoxlcity level, proliferation of lymphocytes and cytokines levels secreted by cultural splenocytes. Results：The data demonstrated that the humoral and cellular immune responses induced by pcDNA3.11L-2/Fc as adjuvant and administrated after HBV preS2 S DNA vaccine 3 days mice were dominant higher than other groups. Conclusion：Genetic adjuvant administrated after Ag priming can enhance its effect.
作者 陈红梅 白雪帆 黄长形 李光玉
机构地区 第四军医大学唐都医院感染病中心
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2006年第10期895-897,共3页 Chinese Journal of Immunology
关键词 HBV DNA疫苗 IL-2 基因佐剂 HBV DNA vaccine IL-2 Genetic adjuvant
分类号 R392.3 [医药卫生—免疫学]
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参考文献9

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中国免疫学杂志
2006年 第10期

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