慢性乙型肝炎患者血清e系统与HBV-DNA含量的关系及临床意义

Relation and clinical meaning of serum e system and HBV-DNA levels in patients with chronic hepatitis B

[目的]探讨慢性乙型肝炎患者血清HBV-DNA的表达水平与e系统的关系及其与肝细胞损害的关系。[方法]用实时荧光定量PCR方法检测乙肝患者血清HBV-DNA,同时用ELISA法检测血清乙肝标志物。[结果]HBeAg(+)组与抗-HBe(+)组HBV-DNA阳性率分别为100%、58.89%,两组HBV-DNA含量比较,差异无显著性(P>0.05);两组肝细胞损害指标ALT、AST、TBA比较,差异有显著性(P<0.05),HBV-DNA含量与肝细胞损害各指标均无相关性。[结论]HBV-DNA的定量检测可更准确地反映体内HBV增殖复制水平,HBV-DNA的含量与肝实质损害程度无关。

[Objective] To explore the relationship between the expression levels of serum HBV - DNA and the serum e system, and the degree of hepatocyte injury in patients with chronic hepatitis B. [Methods] The contents of HBV - DNA were detected by fluorescence quantitative PCR, and the HBV serum marks of immunology by ELISA. [Results] The positive rate of HBeAg（＋） group and anti - HBe（＋） group was respectively 100% and 58.89%, The contents of HBV - DNA copies had no significant difference between two groups （P 〉 0.05）; There had significant difference between the contents of HBV - DNA copies and the severe degree of hepatocyte injury （P 〈 0.05）; The contents of HBV - DNA copies had no correlation with the severe degree of hepatocyte injury （P 〉 0.05）. [Conclusion] The quantitative detection of serum HBV - DNA can accurately react the duplication level of hepatitis virus. The severe degree of hepatocyte injury has not a direct correlation with the contents of HBV - DNA copies.