Aurora-A激酶在人类卵巢癌细胞株中的表达研究

Expression significances of Aurora-A kinase in human ovarian carcinoma cell lines

[目的]了解Aurora-A基因在卵巢癌细胞中的表达情况,寻找显示卵巢癌恶性程度的肿瘤标记物。[方法]采用半定量RT-PCR、Western blot检测卵巢上皮浆液性囊腺癌细胞(HO-8910)与其高转移亚株(HO-8910PM)细胞株Aurora-A在mRNA和蛋白表达情况;借助流式细胞仪观察两种卵巢癌细胞DNA含量,并分析Aurora-A表达的相关性。[结果]两种细胞株HO-8910、HO-8910PM半定量RT-PCR结果发现,Aurora-A/β-actin比值分别为:0.95与0.98(P<0.05);Western blot结果显示Aurora-A/β-actin比值分别为:1.24与1.58(P<0.01)。细胞DNA含量的检测结果,细胞含有四倍体(4N)的细胞比例分别为21.63%和30.15%,其多倍体(>4N)细胞的比例分别为2.20%与7.16%(P<0.01)。[结论]在两种卵巢癌细胞中,Aurora-A基因mRNA与蛋白高表达;两种细胞株Aurora-A基因表达的高低与其细胞DNA含量有相关性,尤其与异倍体的形成有关;Aurora-A基因表达卵巢癌细胞的恶性程度有关。

[Objective] To investigate the expression of Aurora - A in ovarian carcinoma cell lines and to find the tumor marker which show the malignant level of ovarian carcinoma. [Methods] The expressions of Aurora - A in ovarian carcinoma cell lines （HO - 8910, HO - 8910PM） in mRNA, protein level were examined by RT - PCR and Western - blot; and the association of Aurora - A expression with DNA content in two ovarian carcinoma cell lines was analyzed by flow cytometry. [Results] In HO - 8910 and HO - 8910 PM, the ratios of Aurora - A/β - actin were 0.95 and 0.98 respectively （P 〈 0.05） by RT - PCR; while 1.24 and 1.58 by western blot （P 〈 0.01）. The proportion of cells with 4N DNA content in HO - 8910 and HO - 8910PM were 21.63% and 30.15% respectively, the proportion with 〉 4N DNA content were 2.20% and 7.16% respectively （P 〈 0.01）. [Conclusion] Aurora - A was overexpressed in ovarian carcinoma cell lines in mRNA and protein level. The overexpression of Aurora - A in two ovarian carcinoma cell lines has correlation to the DNA content especially with the formation of the heteroploid; The expression of Aurora - A relationed to the malignancy level of ovarian carcinoma.