银杏叶提取物对脂多糖介导的血管内皮细胞低密度脂蛋白受体LOX-1表达的影响

Effect of GBE50 on LPS-induced LOX-1 expression in HUVECs

[目的]观察银杏叶提取物(GBE50)对脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)氧化低密度脂蛋白受体(lectin like oxidized low density lipoprotein receptor,LOX-1)表达的影响。[方法]应用LPS刺激体外培养的HUVECs 24h。RT-PCR方法检测(lectin like oxidized low density lipoprotein receptor mRNA,LOX-1 mRNA)表达水平;采用蛋白质印迹分析检测LOX-1蛋白表达水平。[结果]LPS(1μg/mL)上调LOX-1 mRNA和蛋白表达水平,GBE50(40μg/mL)轻度抑制LPS介导的LOX-1 mRNA(P<0.005)和蛋白表达增加(但没有统计学意义,P>0.05),GBE50(80μg/mL)及NF-κB抑制剂CAPE(20μg/mL)明显抑制LOX-1 mRNA和蛋白表达上调(P<0.01)。[结论]GBE50可能通过抑制LOX-1表达,在防治动脉粥样硬化中发挥作用。

[Objective] To investigate the effect of Ginkgo biloba extract 50 （GBE50） on LPS - induced LOX - 1 expression in HUVECs. [Metheod] HUVECs were incubated by purified LPS for 24 h. LOX - 1 mRNA were measured by RT - PCR, the protein expression of LOX - 1 were detected by Western blot. [Results] LPS（1 μg/mL） upregulated LOX - 1 mRNA and protein expression, GBE50（40 μg/mL） suppressed LPS - induced LOX - 1 mRNA expression（P 〈 0.05） and protein expression（P 〈 0.05） GBE50 （80 μg/mL）and NF - κB inhibitor CAPE both significantly inhibited LOX - 1 mRNA expression and protein expression upregulation. [Conclusion] GBE50 may play a role in anti - atherosclerosis by downregulating LOX - 1 expression.