SDS—PAGE、同工酶等电聚焦和RAPD标记鉴定两系杂交水稻F_1种子纯度对比研究

F_1 Genetic Purity Testing of Two-line Hybrid Rice (Oryza sativa L.) by SDSPAGE of Seed Storage Proteins,Esterase Isoenzyme and RAPD Molecular Markers

目的：探讨利用种子贮藏蛋白SDS—PAGE电泳、酯酶同工酶超薄等电聚焦电泳和RAPD分子标记鉴定五个两系杂交水稻组合F_1种子纯度的可行性。方法和结果：利用SDS—PAGE电泳技术未能找到所试五个组合各自的父本特征蛋白质带；利用酯酶同工酶超薄等电聚焦电泳技术和RAPD分子标记可找到所试五个组合的父母本特征酶带和RAPD标记,但酯酶同工酶的多态性同时也受种子萌发时间的影响。酯酶同工酶超薄等电聚焦电泳和RAPD分子标记可用于所试五个两系杂交水稻组合F_1种子纯度的鉴定。

Objective：To test the F1 genetic purity of five two- line hybrid rice combinations, SDS- PAGE of seed storage proteins, ultrathinlayer isoelectric focusing of esterase isoenzyme and RAPD molecular markers were used in this report. Methods and Results： Male marker bands of proteins could not be found for the five male lines by SDS- PAGE. However, male and female marker bands of esterase isoenzyme and RAPD were found for the combinations. Those male and female marker bands could be used to test the F1 genetic purity of five two- line hybrid rice combinations tested in this report. It was also found that the polymorphism of esterase isoenzyme could be influenced by the age of seedlings from which enzymes were extracted.