两种从土壤中提取DNA方法的比较

Comparison of Two DNA-Extraction from Soil

为土壤微生物多样性研究选取理想的DNA提取方法,该文比较了直接法和间接法从土壤中提取微生物总DNA的效果。结果表明：直接法提取量大,每克土壤提取到DNA约10．26μg,而间接法仅提取到0．55μg,直接法DNA提取量约为间接法的19倍。直接法得到的DNA包含细菌种群较间接法丰富,DGGE分析结果显示,二者的条带数分别为35条和28条,占检测条带总数的92．1%和78．9%。间接法提取到DNA纯度较直接法高,不需纯化即可用于PCR扩增和BamHⅠ酶切反应。

To provide an ideal DNA-extraction aproach for mlecular lechnique in microbial diversity research,the effect of direct and indirect method was compared in this paper.Results revealed that direct extraction often had higher efficiency,about 10.26μg DNA could be extracted form one gram soil on average,which was approximal 19 times as much as that extracted by indirect method.In addition,Directed DNA concloded more diverse bacterial species.DGGE pattem showed that of all the 38 detected bands,direct and indirect method detected 35 and 28 bands respectively,accounting for 92.1% and 78.9% of the total.However,DNA extracted by direct approach was so impure that PCR reaction and Bam H I digestion can＇t conduct except purification.This paper offered an optional DNA extraction for scholars in soil microbial research.