摘要
目的 检测喉鳞状细胞癌（LSCC）中Survivin、增殖细胞核抗原（PCNA）基因蛋白的表达特征及临床意义。方法 采用免疫组织化学SAN2法对76例LSCC及20例喉声带息肉组织进行染色，检测Survivin、PCNA基因蛋白的表达，并分析两者与喉癌临床特征的关系。结果 ①Survivin、PCNA基因蛋白在LSCC中的表达阳性率分别为60．5％（46／76）和76．3％（58／76），均显著高于声带息肉组织阳性表达率（P〈0．05）。②晚期（Ⅱ～Ⅲ期）、组织学分级2～3级和伴颈淋巴结转移的喉癌组织中Survivin、PCNA阳性表达率均显著高于早期（Ⅰ～Ⅱ期）、组织学分级1级和无颈淋巴结转移的喉癌组织（P〈0．05）。③喉癌组织中Survivin蛋白表达阳性的PCNA增殖指数明显高于Survivin蛋白表达阴性者，PCNA的表达与Survivin的表达显著相关（P〈0．05）。结论 Survivin、PCNA的异常表达在LSCC的发生发展过程中起重要作用，PCNA可反映LSCC的增殖状况，两者临床上可作为LSCC病理诊断及判断预后的参考指标。
Objective To examine the expressions of Survivin protein, proliferating cell nuclear antigen （PCNA） and their clinical significance in laryngeal squamous cell carcinoma （LSCC）. Methods Immunohistochemical SABC method was used to test the expressions of PCNA, Survivin gene in paraffin-embeded specimens from 76 LSCC and 20 vocal cord polyps. Results ① The positive expression rates of Survivin and PCNA in LSCC were stronger than those in vocal cord polyps. ② The expressions of Survivin and PCNA in patients of stages Ⅲ～Ⅴ, poor differentiation and with cervical lymphatic metastasis were stronger than those of stages Ⅰ～Ⅱ , well differentiation and without cervical lymphatic metastasis respectively （ P 〈0.05）. ③ The expressions of Survivin protein and PCNA were highly correlated （ P 〈0.05）. Conclusion The abnormal expressions of Survivin and PCNA may play an important role in the oncogenesis and development of LSCC and can be used as diagnostic and prognostic predictors of this tumor.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2006年第5期326-329,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
