我国斑鳜不同群体mtDNA控制区序列的遗传变异

Genetic variation of the mitochondrial DNA control region among 5 populations of Siniperca scherzeri Steindachner in China

采用PCR技术扩增了鸭绿江、长江、钱塘江、闽江、西江5个群体36尾斑鳜(Siniperca scherzeriSteindachner)mtDNA控制区的基因序列。在长度818 bp片断序列中共检测到112个多态性核苷酸位点,具有20种单倍型,不同地理群体的单倍型表型不同,鸭绿江、钱塘江群体中含有多个特异性的核苷酸位点。用MEGA 3.0构建了NJ分子树,鸭绿江、长江、钱塘江、西江群体内的个体均能单独聚成群,而闽江群体未能单独成群,个体分别与长江、西江群体聚在一起。这些表明我国斑鳜不同群体间有较明显的遗传分化。

The mitochondrial DNA control regions of Siniperca scherzeri Steindachner collected from the Yalujiang River, Yangtze River, Qiantangjiang River, Minjiang River and Xijiang River were amplified with PCR technique and sequenced. The result showed 112 nucleotide sites were variable among 818 bp length of homologous sequence and 20 haplotypes were found in all 36 individuals. Each population owned the different haplotypes, some population specific sites were also found in the populations from the Yalujiang River and Qiantangjiang River. Molecular tree constructed by NJ method showed all the individuals from the Yalujiang River, Yangtze River, Qiantangjiang River and Xijiang River populations, respectively, could be clustered as one group independently, except that the individuals from the Minjiang River were mixed with those of the Yangtze River and Xijiang River. These showed there were significant genetic differences among the 5 populations of S. scherzeri in China.