精子膜蛋白在异精雌核发育银鲫中的作用

Effect of the sperm proteins in allogynogenetic Carassius auratus gibelio

通过对雄性银鲫的新鲜精液进行分级抽提得到精子的不同组份,包括精浆、精头的膜、鞭毛和脱膜精头等,在荷包红鲤和雌性银鲫杂交时加入其中的可溶性精头膜蛋白组分,研究精子膜蛋白在雌核发育银鲫异源受精中的作用。研究发现,加入同源精子膜蛋白后的雌核发育银鲫异源受精卵的孵化率比对照组低很多,并且在子代培育过程中,发现了生长速度快、表型和父本相似、基因型基本和父本基因型相同的特殊子代。在某种程度上表明:精子膜蛋白可能是雌核发育银鲫卵子进行雄核发育的关键因素。

The sperm proteins were extracted from the fresh spermatic fluid of male gynogenetic silver crucian carp （ Carassius auratus gibelio ）, and various fractions were collected, the fractions include sperm plasma, sperm head membrane, flagella, and demembranated sperm head,etc, in order to reveal the roles of the homologous sperm head membrane proteins to allogenetic fertilization in gynogenetic silver crucian carp. The soluble protein components of the sperm head membrane were, mixed with sperms of purice red carp（ Cyprinus carpio var vuyuanensis ） and then this cocktail was fertilized to eggs of silver crucian carp. The obtained results show that the hatching ratio of the experimental group is lower than that of the control group. Furthermore, during the filial generation breeding, two special individuals which grow faster were found, the special individuals have the similar phenotype and genotype to their father. To a certain degree, sperm head membrane maybe is the key factor to the androgenesis of the gynogenetic silver crucian carp eggs.