5-HT1D基因多态与共患破坏性行为障碍的儿童注意缺陷多动障碍的相关性

Association between serotonin 1D gene polymorphisms and attention deficit hyperactivity disorder comorbid or not comorbid disruptive behavior disorder

目的:探讨共患和不共患破坏性行为障碍(DBD)的儿童注意缺陷多动障碍(ADHD)与5-HT1D受体基因(HTR1D)1350T>C和1236A>G两种多态之间的关联。方法:检测90个共患DBD的ADHD核心家系和182个不共患DBD的ADHD核心家系的1350T>C和1236A>G两种多态,并分别进行传递不平衡(TDT)检验和单体型分析。结果:在共患DBD的家系中,1350T等位基因(χ2=3.67,P=0.055)和G/T单体型(χ2=3.84,P=0.050)优先传递,1350C等位基因(χ2=3.67,P=0.055)和G/C单体型(χ2=5.22,P=0.022)传递减少。在不共患DBD的家系中未发现任何等位基因和单体型的传递不平衡现象。结论:ADHD共患DBD与否在HTR1D受体基因多态水平存在遗传差别,遗传在共患DBD的ADHD中具有更重要的作用。

Objective： To investigate the relationship between two HTR1 D gene polymorphisms, that is 1350T 〉 C and 1236A 〉 G polymorphisms, and attention deficit hyperactivity disorder （ADHD） comorbid or not comorbid disruptive behavior disorder （DBD）. Methods： Blood samples were taken from 90 trios with probands of ADHD comrbid DBD and 182 trios with probands of ADHD not comorbid DBD. DNA was extracted. 1350T 〉 C and 1236A 〉 G were genotyped by restriction fragment length polymorphism analysis. Transmit/disequilibrium test and haplotype analysis were used to test the association of the two polymorphisms of HTR1 D gene and ADHD comorbid or not comorbid disruptive behavior disorder （DBD） separately. Results ： 1350T allele （χ^2 = 3.67, P = 0. 055 ） and G/T haplotype （ χ^2 = 3.84, P = 0. 050 ） were overtransmitted, while 1350C allele （χ^2 = 3.67, P = 0. 055 ） and G/C haplotype （χ^2 = 5.22, P = 0. 022） were undertransmitted to probands of ADHD with DBD. No biased transmission of any allele and haplotype was found in families with probands of ADHD without DBD. Conclusion： ADHD comorbid or not comorbid DBD are different at the level of HTR1D gene polymrohisms of 1350T 〉 C and 1236A 〉 G. The current results indicate that ADHD with DBD has more heritable backgrounds when compared with ADHD without DBD.