rhG-CSF增加实验性脑缺血周围区微管相关蛋白的表达

rhG-CSF enhances expression of microtubule-associated protein 2 in peri-infarct zone after focal cerebral ischemia:An experimental study

目的研究重组人粒细胞集落刺激因子(Reeomhinant human gmnulocyte colony-stimulating factor,rhG-CSF)对脑梗死周围区的微管相关蛋白(Microtubule-associated protein 2,MAP-2)表达的影响。方法用线栓法建立大鼠大脑中动脉缺血再灌注模型,观察rhG-CSF对神经功能缺损评分的影响；应用半定量逆转录一聚合酶链式反应(RT-PCR)方法和免疫组织化学方法分别测定梗死周围区MAP-2的mRNA和蛋白表达水平的变化。结果与对照组相比,治疗组的神经功能缺损评分较低,脑缺血周围区MAP-2的mRNA和蛋白表达增高。结论rhG-CSF可以促进脑缺血周围区的微管相关蛋白的表达,从而减轻脑缺血再灌注损伤。

Objective To investigate the effects of recombinant human granulocyte colony-stimulating factor （rhG-CSF） on microtubule-associated protein 2 （MAP-2） expression of periinfaretion after focal cerebral ischemia.Methods Focal brain ischemia in rats was performed with the intraluminal filament occlusion.The effects of rhG-CSF were evaluated by neurological severity score （NSS）.Reverse transcriptional polymerase chain reaction （RT-PCR） and immunohistochemical techniques were used to detect MAP-2 expression in the peri-infarct zone of the brain. Results Compared with controUed group rhG- CSF significantly reduced the neurological severity scores,increased the expression of MAP-2. Conclusion The rhG-CSF may enhance MAP-2 expression and thus alleviate the brain damage after ischemia/ reperfusion lesion.