2种病原菌感染海鳗后外周血白细胞的病理变化

Changes in Number and Structure of Peripheral Leucocytes of Pike Eel Infected with Two Species of Fish Bacterial Pathogens

用鳗弧菌(Vibrio anguillarum)和嗜水气单胞菌(Aeromonas hydrophila)分别感染海鳗(Muraenesoxcinereus),48 h后取其外周血液,瑞-吉(Wright-Giemsa)氏染色,光镜下观察,同时对其外周血液中的免疫细胞数量变化进行测定。鳗弧菌和嗜水气单胞菌感染组的单核细胞的伪足样突起与对照组比较明显增多,胞质内空泡增多、变大,数量明显增多,差异极显著;嗜中性粒细胞核多呈长杆状,数量与对照组比较明显增多,差异极显著;淋巴细胞伪足样突起明显,中、大型淋巴细胞较多,数量与对照组相比明显减少,差异极显著;血栓细胞的数量明显下降,差异极显著。

The Pike Eel （Muraenesox cinereus ） was infected separately by Vibrio anguillarum and Aeromonas hydrophila for the observation of changes in the number and structure of leucocytes. The numbers of lymphocytes, neutrophils, monocytes and the thrombocytes from the peripheral blood were measured 48 h after the infection. In both V. anguillarum and A. hydrophila infected Pike Eels, the monocytes had obviously vacuolated cytoplasm and pseudopodia, with its number increased remarkably when compared to the control. Most neutrophils had rod nucleus in the infected Pike Eels, and their number increased remarkably compared to the control. The lymphocytes had distinct pseudopodia, and much more large and medium-sized lymphocytes were present, while their number decreased significantly when compared to the control. The number of the thrombocytes also decreased drastically in the infected Pike Eels