3种爬行动物消化道嗜银细胞的分布密度和形态学观察

Distribution and Morphology of Argyrophil Cells in Digestive Tract of Three Species of Reptile

为了探索爬行动物消化道内分泌细胞的分布规律和分泌类型,以改良龙桂开浸银法对中华鳖(Trionyx sinensis)、巴西彩龟(Truchemys scripta)和无蹼壁虎(Gekko swinhonis)的消化道进行了染色和切片观察。结果表明,3种动物消化道从食道到大肠均有嗜银细胞分布,均在胃幽门有突出的分布密度高峰,分别在小肠末段和大肠始段、大肠中段有次分布密度高峰,巴西彩龟在食道还有第三分布密度高峰;嗜银细胞多数为毛笔头形、高脚杯状、长梭形、椭圆形和锥体形等,多数嗜银细胞有明显的突起伸向管腔方向,少数嗜银细胞的突起伸向固有膜和周围细胞,突起处常可见明显的分泌颗粒。提示消化道内分泌细胞兼有内分泌与外分泌两种功能。

By using the improved Long＇ s silver impregnation method, the distribution and morphology of argyrophil cells in the digestive tract of Trionyx sinesis, Truchemys scripta and Gekko swinhonis were observed. The results show that argyrophil cells distribute throughout the digestive tract of three kinds reptile, from esophagus to rectum, with the highest density in the pars pyloric, the second highest in the last part of intestinum tenue and froerpart, middle part of intestinum crassum, respectively. Moreover, high density of argyrophil cells is also observed in the esophagus of Truchemys scripta. Argyrophil cells appear in the shape of goblet, brush pen head, pyramid, oval, or long shuttle. Most argyrophil cells possess processes stretching to the lumen, while a minority stretching their processes to the lamina porpria. Some processes are surrounded by secretory granules. Our observations suggest that argyrophil cells may have both exocrine and endocrine functions.