黑木耳多糖的提取及降血糖作用

Extraction of Polysaccharide from Auricularia auricular and its Hypoglycemia Activity

利用纤维素酶(EC 3.2.1.4)和蛋白酶(EC 3.4.14.9)从黑木耳当中提取出了2种多糖,相对分子质量分别为3.17×105和1.83×105。以正常小鼠和糖尿病小鼠为对象,对黑木耳多糖的降血糖功能进行了研究,使用剂量为100,200,400 mg/kg(以体重计)。结果表明,当质量分数在200 mg/kg以上,黑木耳多糖能够显著降低糖尿病小鼠的血糖值,但对正常小鼠的血糖值没有影响。黑木耳多糖能够增加糖尿病小鼠的糖耐量。

Two novel polysaccharides （APE I and APE II） were extracted and purified from Auricularia auricular, by cellulose （E. C. 3.2.1.4） and protease （E. C. 3.4.14.9）. The average molecular weights was 3.17 × 10^5 and 1.83 × 10^5, respectively. Furthermore, the hyperglycemia mice were used as a model to investigate the hypoglycemia activity of APE I, and the normal mice was as control. The results indicated that when more than 200 mg/kg body weight APE I was feed to the mice, the blood glucose of hyperglycemia mice were decreased significantly. However, no effect was observed for the control mice. On the other hand, APE I also could enhance blood glucose tolerance and decrease area under glucose curve of the hyperglycemia mice.