急性髓细胞白血病30例免疫分型特点分析

Characteristic analysis of immunological classification of 30 acute myeloid leukemia

目的探讨急性髓细胞白血病(AML)抗原表达特征及免疫分型的意义。方法采用流式细胞术对30例AML进行免疫表型分析。结果(1)29例AML表达髓系抗原,阳性率分别为CDl373.3%,CDl446.7%,CD3323.3%;其中16例AML伴淋巴系抗原表达,分别为CD1050%,CD513.3%,CD76.7%。(2)30例AML中,未分化型3.3%,单纯型43.3%,变异型53.3%。(3)CDl4在M5型AML中高表达。(4)CDl0表达阳性者CR率高于阴性者(P=0.046)。结论白血病免疫分型能确诊某些特殊类型的白血病,对于急性髓细胞白血病的诊断、治疗及预后判断均有重要价值。

Objective To study the characteristics of antigen expression and its clinical significance in acute myeloid leukaemia （AML）. Methods The immunological phenotypes in 30 patients with AML were assayed by flow cytometry. Results （1） Myeloid antigen was expressed in 29 cases, with CD1373.3%, CD1446.7 % and CD33 23.3%, respectively. Meanwhile 16 cases accompained lymphocyto antigen expression （CD10 50%, CDs 13.3%, and CD7 6.7%）. （2） The undifferentiated phenotype account for 3.3%, simple phenotype 43.3%, and variant phenotype 53.3%. （3） CD14 antigen was highly expressed in Ms subtype. （4） CR rate of the patients with CD10 positive was significantly higher than that of those with CD10 negative （P= 0. 046）. Conclusion Leukemia immunophenotyping could classify some particular types of myeloid leukemia with important value in diagnosis, treatment and predicting prognosis of acute myeloid leukemia.