AFM观察小鼠心肌核DNA片段的基因组合形成“基因系”的系统性和垃圾DNA的相互作用

AFM Observation of Mouse Cardiac Nuclear Gene Combination Forming Gene Lineage's Systematicness and Junk DNA's Interaction

用原子力显微镜(AFM)直接观察、小白鼠心肌等组织的核DNA片段的基因体外转录等多种实验技术组合,通过AFM观察到心肌核DNA片段上的基因,处于垃圾DNA的“转录平台”上,在体外转录过程中,由n(n=3、4等)个活性基因节,对应的n-1个“基因间隔”,依特定的排列组合分别形成n(n=3、4等)种大小不同的“基因系”,各“基因系”中的基因节同时转录,分别形成对应nRNA(n=9、12等)链状复合体,nRMA链状复合体分别与对应基因系的单链DNA两边的“接口”相联。核内对应nmRNA数量减少,形成负反馈效应后,使nRNA从对应“基因系”上迅速解离下来,核内经“转录后修饰”形成对应nmRNA链状复合体。该复合体主要在核内,处于垃圾DNA的“翻译平台”上进行蛋白质翻译,并在核内加工修饰形成有活性蛋白质。本工作展示了未来运用AFM观察生物学反应、研究核基因转录与调控的分子机理、基因组合形成基因系的系统性和垃圾DNA的相互作用的前景。

Direct atomic force microscopy （AFM） observation and in vitro transcription techniques were used to investigate that mouse cardiac nuclear genes occur on transcription platform of junk DNA. During in vitro transcription, n （n=3 or 4） active gene knots and n-1 gene intervals are put in special permutation and combination to form n （n=3 or 4） different sizes of gene lineage. Different gene knots simuhaneouslv transcribe n mRNA （n=9 or 12） chain complexes, n mRNA （n=9 or 12） chain complexes are respectively linked with two ends of adaptors of related single chain DNAs. If the number of n mRNA （n=9 or 12） decreased, n mRNA （n=9 or 12） may be dissociated from gene lineages and form n mRNA （n=9 or 12） chain complexes through post-transcription modification. These complexes mainly occur in nuclei and are translated into proteins on translation platform, then these proteins are modified to form active proteins. This work shows that direct AFM observation can be used to investigate gene transcription and regulation, formation of gene lineage and Junk DNA interaction.