凝集素-胃癌单克隆抗体血清诊断的方法及临床应用

Application of Lectin - McAb in serum diagnosis of gastric cancer

作者根据鼠抗MG7胃癌单克隆抗体相应抗原能与刀豆素A专一性结合的特性,建立了一种凝集素-单抗ELISA检测方法,并用于MG7相应抗原的血清和胃液检测。结果显示,血清MG7相应抗原检测胃癌阳性率为41.1％(37/90);肿瘤切除后10～20d阳性率降至5.9％(1/17).良性疾病患者为4.4％(4/90).胃液中相应抗原检测胃癌阳性率为40.4％(21/52),良性胃病患者假阳性率为3.2％(1/31).故认为MG7相应抗原的检测对胃癌的诊断具有一定帮助。

A Lectin-McAb method was established and emploied to investigate the corresponding antigen of MG7(a monoclonal antibody against gastric cancer) in serum and gastric juice of patients with gastric cancer and benign di -seases. The positive rates were found to be 41.1% (37/90) in sera and 40.4% (21/52) in gastric juice of patients with gastric cancer. In sera and gastric juice of patients with benign diseases, the positive rates were only found to be 4.4% ( 4/90) and 3.2% (1/31). The corresponding antigen of MG7 was also detected in sera of 17 patients with gastric cancer, who underwent gastrorectomy. Only one positive case was found in them. It suggest that determination of the corresponding antigen of MG7 be useful in the diagnosis of gastric cancer.